English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Vibrio cholerae  and  Vibrio parahaemolyticus  are common diarrheal pathogens of great public health concern. A multiplex TaqMan-based real-time PCR assay was developed on the BD MAX platform; this assay can simultaneously detect and differentiate  V. cholerae  and  V. parahaemolyticus  directly from human fecal specimens. The assay includes two reactions. One reaction, BDM-VC, targets the gene  ompW , the cholera toxin (CT) coding gene  ctxA , the O1 serogroup specific gene  rfbN , and the O139 serogroup specific gene  wbfR  of  V. cholerae . The other, BDM-VP, targets the gene  toxR  and the toxin coding genes  tdh  and  trh  of  V. parahaemolyticus . In addition, each reaction contains a sample process control. When evaluated with spiked stool samples, the detection limit of the BD MAX assay was 195–780 CFU/ml for  V. cholerae  and 46–184 CFU/ml for  V. parahaemolyticus , and the amplification efficiency of all genes was between 95 and 115%. The assay showed 100% analytical specificity, using 63 isolates. The BD MAX assay was evaluated for its performance compared with conventional real-time PCR after automated DNA extraction steps, using 164 retrospective stool samples. The overall percent agreement between the BD MAX assay and real-time PCR was ≥ 98.8%; the positive percent agreement was 85.7% for  ompW , 100% for  toxR / tdh , and lower (66.7%) for  trh  because of a false negative. This is the first report to evaluate the usage of the BD MAX open system in detection and differentiation of  V. cholerae  and  V. parahaemolyticus  directly from human samples.

frontiers in cellular and infection microbiology

Development of a Rapid and Fully Automated Multiplex Real-Time PCR Assay for Identification and Differentiation of Vibrio cholerae and Vibrio parahaemolyticus on the BD MAX Platform