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Vibrio parahaemolyticus  is a leading seafood-borne pathogen that can cause acute gastroenteritis and even death in humans. In aquatic ecosystems, phages constantly transform bacterial communities by horizontal gene transfer. Nevertheless, biological functions of prophage-related genes in  V. parahaemolyticus  remain to be fully unveiled. Herein, for the first time, we studied one such gene  VpaChn25_0724  encoding an unknown hypothetical protein in  V. parahaemolyticus  CHN25. This gene deletion mutant Δ VpaChn25_0724  was constructed by homologous recombination, and its complementary mutant Δ VpaChn25_0724 -com was also obtained. The Δ VpaChn25_0724  mutant exhibited a sever defect in growth and swimming motility particularly at lower temperatures. Biofilm formation and cytotoxicity capacity of  V. parahaemolyticus  CHN25 was significantly lowered in the absence of  VpaChn25_0724 . Comparative secretomic analysis revealed an increase in extracellular proteins of Δ VpaChn25_0724 , which likely resulted from its damaged cell membrane. Comparison of transcriptome data showed twelve significantly altered metabolic pathways in Δ VpaChn25_0724 , suggesting inactive transport and utilization of carbon sources, repressed energy production and membrane biogenesis in  ΔVpaChn25_0724 . Comparative transcriptomic analysis also revealed several remarkably down-regulated key regulators in bacterial gene regulatory networks linked to the observed phenotypic variations. Overall, the results here facilitate better understanding of biological significance of prophage-related genes remaining in  V. parahaemolyticus .

Prophage-Related Gene VpaChn25_0724 Contributes to Cell Membrane Integrity and Growth of Vibrio parahaemolyticus CHN25