English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Overuse or abuse of antibiotics has undoubtedly accelerated the increasing prevalence of global antibiotic resistance crisis, and thus, people have been trying to explore approaches to decrease dosage of antibiotics or find new antibacterial agents for many years. Antimicrobial peptides (AMPs) are the ideal candidates that could kill pathogens and multidrug-resistant bacteria either alone or in combination with conventional antibiotics. In the study, the antimicrobial efficacy of mud crab  Scylla paramamosain  AMPs Sphistin and Sph 12−38  in combination with eight selected antibiotics was evaluated using a clinical pathogen,  Pseudomonas aeruginosa . It was interesting to note that the  in vitro  combination of rifampicin and azithromycin with Sphistin and Sph 12−38  showed significant synergistic activity against  P. aeruginosa . Moreover, an  in vivo  study was carried out using a mouse model challenged with  P. aeruginosa , and the result showed that the combination of Sph 12−38  with either rifampicin or azithromycin could significantly promote the healing of wounds and had the healing time shortened to 4–5 days compared with 7–8 days in control. The underlying mechanism might be due to the binding of Sphistin and Sph 12−38  with  P. aeruginosa  lipopolysaccharides (LPS) and subsequent promotion of the intracellular uptake of rifampicin and azithromycin. Taken together, the significant synergistic antibacterial effect on  P. aeruginosa in vitro  and  in vivo  conferred by the combination of low dose of Sphistin and Sph 12−38  with low dose of rifampicin and azithromycin would be beneficial for the control of antibiotic resistance and effective treatment of  P. aeruginosa -infected diseases in the future.

frontiers in cellular and infection microbiology

The Synergistic Effect of Mud Crab Antimicrobial Peptides Sphistin and Sph12−38 With Antibiotics Azithromycin and Rifampicin Enhances Bactericidal Activity Against Pseudomonas Aeruginosa