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An important component in host resistance to malaria infection are inherited mutations that give rise to abnormalities and deficiencies in erythrocyte proteins and enzymes. Understanding how such mutations confer protection against the disease may be useful for developing new treatment strategies. A mouse ENU-induced mutagenesis screen for novel malaria resistance-conferring mutations identified a novel non-sense mutation in the gene encoding porphobilinogen deaminase (PBGD) in mice, denoted here as  Pbgd   MRI 58155 . Heterozygote  Pbgd   MRI 58155  mice exhibited ~50% reduction in cellular PBGD activity in both mature erythrocytes and reticulocytes, although enzyme activity was ~10 times higher in reticulocytes than erythrocytes. When challenged with blood-stage  P. chabaudi , which preferentially infects erythrocytes, heterozygote mice showed a modest but significant resistance to infection, including reduced parasite growth. A series of assays conducted to investigate the mechanism of resistance indicated that mutant erythrocyte invasion by  P. chabaudi  was normal, but that following intraerythrocytic establishment a significantly greater proportions of parasites died and therefore, affected their ability to propagate. The  Plasmodium  resistance phenotype was not recapitulated in  Pbgd -deficient mice infected with  P. berghei , which prefers reticulocytes, or when  P. falciparum  was cultured in erythrocytes from patients with acute intermittent porphyria (AIP), which had modest (20–50%) reduced levels of PBGD. Furthermore, the growth of  Pbgd -null  P. falciparum  and  Pbgd -null  P. berghei  parasites, which grew at the same rate as their wild-type counterparts in normal cells, were not affected by the PBGD-deficient background of the AIP erythrocytes or  Pbgd -deficient mice. Our results confirm the dispensability of parasite PBGD for  P. berghei  infection and intraerythrocytic growth of  P. falciparum , but for the first time identify a requirement for host erythrocyte PBGD by  P. chabaudi  during  in vivo  blood stage infection.

frontiers in cellular and infection microbiology

Host Porphobilinogen Deaminase Deficiency Confers Malaria Resistance in Plasmodium chabaudi but Not in Plasmodium berghei or Plasmodium falciparum During Intraerythrocytic Growth