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The Mkt1–Pbp1 complex promotes mating-type switching by regulating the translation of  HO  mRNA in  Saccharomyces cerevisiae . Here, we performed  in vivo  immunoprecipitation assays and mass spectrometry analyses in the human fungal pathogen  Cryptococcus neoformans  to show that Pbp1, a poly(A)-binding protein-binding protein, interacts with Mkt1 containing a PIN like-domain. Association of Pbp1 with Mkt1 was confirmed by co-immunoprecipitation assays. Results of spot dilution growth assays showed that unlike  pbp1  deletion mutant strains,  mkt1  deletion mutant strains were not resistant to heat stress compared with wild-type. However, similar to the  pbp1  deletion mutant strains, the  mkt1  deletion mutants exhibited both, defective dikaryotic hyphal production and reduced pheromone gene ( MF α1) expression during mating. In addition, deletion of  mkt1  caused attenuated virulence in a murine intranasal inhalation model. Taken together, our findings reveal that Mkt1 plays a crucial role in sexual reproduction and virulence in  C. neoformans .

Pbp1-Interacting Protein Mkt1 Regulates Virulence and Sexual Reproduction in Cryptococcus neoformans