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Yersinia enterocolitica  encodes a chromosomal AmpC β-lactamase under the regulation of the classical  ampR-ampC  system. To obtain a further understanding to the role of low-molecular-mass penicillin-binding proteins (LMM PBPs) including PBP4, PBP5, PBP6, and PBP7, as well as NagZ and AmpR in  ampC  regulation of  Y. enterocolitica , series of single/multiple mutant strains were systematically constructed and the  ampC  expression levels were determined by  luxCDABE  reporter system, reverse transcription-PCR (RT-PCR) and β-lactamase activity test. Sequential deletion of PBP5 and other LMM PBPs result in a continuously growing of  ampC  expression level, the β-lactamse activity of quadruple deletion strain YEΔ4Δ5Δ6Δ7 ( pbp4, pbp5, pbp6 , and  pbp7  inactivated) is approached to the YEΔD123 ( ampD1, ampD2 , and  ampD3  inactivated). Deletion of  nagZ  gene caused two completely different results in YEΔD123 and YEΔ4Δ5Δ6Δ7, NagZ is indispensable for YEΔ4Δ5Δ6Δ7  ampC  derepression phenotype but dispensable for YEΔD123. AmpR is essential for  ampC  hyperproduction in these two types of strains, inactivation of AmpR notable reduced the  ampC  expression level in both YEΔD123 and YEΔ4Δ5Δ6Δ7.

Role of Low-Molecular-Mass Penicillin-Binding Proteins, NagZ and AmpR in AmpC β-lactamase Regulation of Yersinia enterocolitica