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Since the first report of  bla  NDM-1 , 16  bla  NDM  variants have been identified among Gram-negative bacteria worldwide. Recently, a novel  bla  NDM  variant,  bla  NDM-13 , was identified in the chromosome of an ST101  Escherichia coli  isolate from Nepal. Here we first reported plasmid-mediated  bla  NDM-13  in a carbapenem-resistant  E. coli  ST5138 clinical isolate associated with hospital-acquired urinary tract infection from China.  bla  NDM-13  and  bla  SHV-12  coexisted on the a ~54 Kb self-transferable plasmid. Compared with NDM-1, NDM-13, NDM-3, and NDM-4 had two amino acid substitutions (D95N and M154L), one amino acid substitution (D95N) and one amino acid substitutions (M154L), respectively. Complete plasmid sequencing showed that  bla  NDM-13 -harboring plasmid (pNDM13-DC33) was highly similar to the  bla  NDM-1 -harboring IncX3 plasmid pNDM-HN380, a common  bla  NDM -harboring vector circulating in China. In accordance with the structure of pNDM-HN380, pNDM13-DC33 consists of a 33-kb backbone encoding plasmid replication ( repB ), stability partitioning, and transfer ( tra, trb , and  pil ) functions, and a 21-kb antimicrobial resistance region with high GC content between  umuD  and  mpr  genes. In conclusion, the present study is the first report of a plasmid-encoded  bla  NDM-13  and the complete sequence of a  bla  NDM-13 -harboring plasmid (pNDM13-DC33).  bla  NDM-13  maybe originate from  bla  NDM-1  located on a pNDM-HN380-like plasmid by sequential mutations.

First Report of Complete Sequence of a blaNDM-13-Harboring Plasmid from an Escherichia coli ST5138 Clinical Isolate