English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Shiga toxin-producing  Escherichia coli  (STEC) and  Salmonella  are food-borne pathogens commonly associated with beef, and reliable methods are needed to determine their prevalence in beef and to ensure food safety. Retail ground beef was tested for the presence of  E. coli  O157:H7, STEC serogroups O26, O45, O103, O111, O121, and O145, and  Salmonella  using the DuPont™ BAX® system method. Ground beef (325 g) samples were enriched in 1.5 L of TSB with 2 mg/L novobiocin at 42°C for 18 h, and then evaluated using the BAX® System real-time PCR assays for  E. coli  O157:H7 and STEC suite, and the BAX® System standard PCR assays for  E. coli  O157:H7 MP and  Salmonella . Samples positive for STEC target genes by the BAX® System assays were subjected to immunomagnetic separation (IMS) and plating onto modified Rainbow Agar O157. Enrichments that were PCR positive for  Salmonella  were inoculated into RV broth, incubated for 18 h at 42°C, and then plated onto XLT-4 agar. Presumptive positive STEC and  Salmonella  colonies were confirmed using the BAX® System assays. Results of the BAX® System STEC assays showed 20/308 (6.5%) of samples positive for both the Shiga toxin ( stx ) and intimin ( eae ) genes; 4 (1.3%) for  stx, eae , and O26; 1 (0.3%) for  stx, eae , and O45; 3 (1%) for  stx, eae , and O103; and 1 (0.3%) for  stx, eae , and O145. There were also 3 samples positive for  stx, eae , and more than one STEC serogroup. Three (1.0%) of the samples were positive using the BAX® System real-time  E. coli  O157:H7 assay, and 28 (9.1%) were positive using the BAX® System  Salmonella  assay. STEC O103 and  E. coli  O157:H7 were isolated from 2/6 and 2/3 PCR positive samples, respectively.  Salmonella  isolates were recovered and confirmed from 27 of the 28  Salmonella  PCR positive samples, and a portion of the isolates were serotyped and antibiotic resistance profiles determined. Results demonstrate that the BAX® System assays are effective for detecting STEC and  Salmonella  in beef.

frontiers in cellular and infection microbiology

Detection of Shiga toxin-producing Escherichia coli (STEC) O157:H7, O26, O45, O103, O111, O121, and O145, and Salmonella in retail raw ground beef using the DuPontâ„¢ BAXÂ® system