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The utilization of agricultural products and residues for the production of value-added and biobased products is a highly relevant topic in present research. Due to the natural recalcitrance of lignocellulosic biomass against enzymatic degradation, pretreatments are important requirement for further processes. For the raw material in this study, corn stover (CS) as highly available agricultural residue and maize silage (MS) as model substrate for an ensiled agricultural product were pretreated by steam refining. However, after processing a liquid fraction and fibers are present. Subsequent to steaming the fiber fraction is well characterized. Nonetheless, in depth characterizations of the filtrates are also important for their subsequent utilization. Decreasing molar masses from 7,900 g/mol to 1,100 g/mol for CS filtrates and 100.000–12.900 g/mol for MS filtrates were determined with increasing severity. Due to their proven inhibitory effect on microorganisms weak acids, furans and phenolic compounds within the liquid phased were analyzed. Especially formic acid increases with increasing severity from 0.27 to 1.20% based on raw material for CS and from 0.07 to 0.23% based on raw material for MS. Further GC/MS measurements indicate, that up to 8.25% (CS filtrate) and 5.23% (MS filtrates) of the total peak area is related to inhibitory phenols. Considering the data, detoxification strategies are of non-negligible importance for filtrates after steam refining and should be considered for further research and process or parameter optimizations. An alternative may be the application of milder process conditions in order to prevent the formation of inhibitory degradation products or the dilution of the gained filtrates.

Analytical Characterization and Inhibitor Detection in Liquid Phases Obtained After Steam Refining of Corn Stover and Maize Silage