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A series of new compounds ( 1 - 4 ) based on pyrrole hydrazone Schiff bases were designed and synthesized. The interactions of these new compounds with metal ions and their fluorescent recognition were investigated. All compounds showed “turn-on” fluorescence in the presence of Al 3+  in aqueous solution. Their sensing behaviors with Al 3+  were studied using photophysical experiments, ESI-MS spectrometry analysis,  1 H NMR titration, and DFT calculation. The detection limits of  1 - 4  for the analysis of Al 3+  were found to reach a 10 −8  M level in aqueous solution, which are far lower than the WHO guidelines for drinking water (7.41 mM for Al 3+ ). A high selectivity test paper has been fabricated for Al 3+  detection based on sensor  3 . Theoretical calculations (DFT) have been carried out to elucidate the configuration of  1 - 4  and their Al complexes and rationalize experimental absorption data.

“Turn-On” Fluorescent Biosensors for High Selective and Sensitive Detection of Al3+ Ion