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Diethyl malonate-based fluorescent probe  NE-N   2   H   4   was constructed for monitoring hydrazine (N 2 H 4 ). The novel probe  NE-N   2   H   4   exhibits good properties, such as large Stokes shift (about 125 nm), good selectivity, and low cytotoxicity. This sensing probe  NE-N   2   H   4   can be operated to detect hydrazine in living HeLa cells. Especially after soaking in probe solution, the thin-layer chromatography (TLC) plate could detect the vapor of hydrazine. Therefore, the probe  NE-N   2   H   4   might be used to monitor hydrazine in biosamples and environmental problem.

Diethyl Malonate-Based Turn-On Chemical Probe for Detecting Hydrazine and Its Bio-Imaging and Environmental Applications With Large Stokes Shift