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In this paper, graphene (Gr)/Co 3 O 4  nanocomposite was synthesized and utilized for the development of a novel electrochemical sensor to detect tramadol. Tramadol determination was examined by linear sweep voltammetry, differential pulse voltammetry, cyclic voltammetry, and chronoamperometry on Gr/Co 3 O 4  nanocomposite-modified screen-printed electrode (Gr/Co 3 O 4 /SPE) in phosphate-buffered saline (PBS). Under the optimized condition, the detection limit of tramadol is 0.03 μM (S/N = 3) in the linear ranges of 0.1–500.0 μM. Furthermore, Gr/Co 3 O 4 /SPE was satisfactorily utilized to detect tramadol in tramadol tablet and urine specimens.

A Screen-Printed Electrode Modified With Graphene/Co3O4 Nanocomposite for Electrochemical Detection of Tramadol