English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The compatible solute mannosylglycerate (MG) has exceptional properties in terms of protein stabilization and protection under salt, heat, and freeze-drying stresses as well as against protein aggregation. Due to these characteristics, MG possesses large potential for clinical and biotechnological applications. To achieve efficient MG production,  Corynebacterium glutamicum  was equipped with a bifunctional MG synthase (encoded by  mgsD  and catalyzing the condensation of 3-phosphoglycerate and GDP-mannose to MG) from  Dehalococcoides mccartyi . The resulting strain  C. glutamicum  (pEKEx3  mgsD ) intracellularly accumulated about 111 mM MG (60 ± 9 mg g CDW   −1 ) with 2% glucose as a carbon source. To enable efficient mannose metabolization, the native  manA  gene, encoding mannose 6-phosphate isomerase, was overexpressed. Combined overexpression of  manA  and  mgsD  from two plasmids in  C. glutamicum  resulted in intracellular MG accumulation of up to ca. 329 mM [corresponding to 177 mg g  cell dry weight (CDW)   −1 ] with glucose, 314 mM (168 mg g CDW   −1 ) with glucose plus mannose, and 328 mM (176 mg g CDW   −1 ) with mannose as carbon source(s), respectively. The product was successfully extracted from cells by using a cold water shock, resulting in up to 5.5 mM MG (1.48 g L −1 ) in supernatants. The two-plasmid system was improved by integrating the  mgsD  gene into the  manA -bearing plasmid and the resulting strain showed comparable production but faster growth. Repeated cycles of growth/production and extraction of MG in a bacterial milking-like experiment showed that cells could be recycled, which led to a cumulative MG production of 19.9 mM (5.34 g L −1 ). The results show that the newly constructed  C. glutamicum  strain produces MG from glucose and mannose and that a cold water shock enables extraction of MG from the cytosol into the medium.

Exploring the Potential of Corynebacterium glutamicum to Produce the Compatible Solute Mannosylglycerate