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Rare sugar   D  -allulose as a substitute sweetener is produced through the isomerization of   D  -fructose by   D  -tagatose 3-epimerases (DTEases) or   D  -allulose 3-epimerases (DAEases).   D  -Allulose is a kind of low energy monosaccharide sugar naturally existing in some fruits in very small quantities.   D  -Allulose not only possesses high value as a food ingredient and dietary supplement, but also exhibits a variety of physiological functions serving as improving insulin resistance, antioxidant enhancement, and hypoglycemic controls, and so forth. Thus,   D  -allulose has an important development value as an alternative to high-energy sugars. This review provided a systematic analysis of   D  -allulose characters, application, enzymatic characteristics and molecular modification, engineered strain construction, and processing technologies. The existing problems and its proposed solutions for   D  -allulose production are also discussed. More importantly, a green and recycling process technology for   D  -allulose production is proposed for low waste formation, low energy consumption, and high sugar yield.

Review on D-Allulose: In vivo Metabolism, Catalytic Mechanism, Engineering Strain Construction, Bio-Production Technology