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In the present study, seven substituted 3-benzylquinoxalines were synthesized from substituted phenyl pyruvic acid and o-phenylenediamine. All the synthesized compounds were structurally elucidated by IR, Mass, NMR spectroscopy. Antifungal activity of the synthesized compound was analyzed against a three fungus by agar well diffusion method. All the Compounds showed good antifungal activity against the test fungus.