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This paper reports the chemical compositions and antimicrobial activity of essential oils extracted from Myristica fragrans and Piper betle in individual and combined fractions. Enhanced antimicrobial activity is anticipated when the volatile oils are combined as compounds present in different extracts could complement each other resulting in synergistic effect offering a broader spectrum of microbial resistance. The GC-MS analysis indicates that chemical compositions of M. fragrans and P. betle vary with M. fragrans containing more early-eluting compounds. The combined extract is characterised by compounds present in both extracts, some appear to co-elute in the mixture. The antimicrobial activity of the single and combined extracts against Staphylococcus aureus, Escherichia coli and Aspergillus flavus were evaluated. P. betle demonstrates stronger antimicrobial activity than M. fragrans; the combined extract exhibit improved performance especially on A. flavus.

Combining Essential Oils of Piper betle and Myristica fragrans for Enhanced Antimicrobial Properties