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A method has been developed which enabled 100 white clover cultivars to be screened and ranked for improved nutritive value. Our objective was to identify cultivars having protein which was relatively insoluble and resistant to rumen degradation. The methods were developed for fresh forages, so that the results were applicable to the grazing animal. The clover was harvested and minced to give a particle size distribution similar to material chewed by ruminants during eating. Crude protein solubility was estimated by measuring nitrogen (N) loss from minced clover incubated in nylon bags in the rumen of a cow. This required the optimal amount of dry matter (DM) and incubation times to be determined, variation between animals to be defined and repeatability of measurements established. Crude protein degradation was measured by incubating minced clover with buffered rumen bacteria in vitro and determining net conversion of plant N to ammonia N. Nitrogen loss to degradation and nutritive value, and a knowledge of white clover ingestion, digestion and utilization by ruminants. The chemical composition of white clover is implicit in such an understanding, but plant growth habits and morphology were also important. This paper outlines the screening procedures and the selection of cultivars most likely to benefit ruminants. The methods were developed with a single cultivar of white clover grown under glass and are applicable to other forages such as grasses, tannin containing legumes and conserved feeds. However, in each instance some of the criteria for optimal nutritive value will differ in accordance with the characteristics of the forage under investigation, and require some modification to the screening process. Nutritive value of white clover

Screening white clover cultivars- for improved nutritive value - development of a method