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Objective: Food addiction is an eating disorder affecting the behavioral and neurological condition associated with BMI (Body mass index), BED (binge eating disorder) and obesity in human being. High-calorie foods, especially sugar, have an addictive potential. The conventional treatment processes involving cognitive behavioral therapy, mental health treatments and intake of drugs have acute side effects. The objective of this study was to characterize a high calorie natural food honey which has been reported to have addictive behavior, and generate peptides from the protein using enzyme. Methods: Protein from honey was concentrated by ultrafiltration purified by ion exchange chromatography, characterized by SDS-PAGE, isoelectric focusing, sequencing and identified by MALDI-TOF/MS analysis. Results: Ultrafiltration was found to significantly concentrate the protein and chromatographic techniques resulted in purification of protein to homogeneity. The protein having molecular weight of 55 kDa was found to have a pI of 5.5 and has hydrophilic N terminal sequence. The protein was identified as Major royal jelly protein 1, most abundant protein present in honey. Peptides were generated with high antioxidant property. Conclusion: Protein is a major biomolecule in honey exhibiting the biological activities in honey. The characterization of protein in this study helps to get idea of the molecular character so that further studies on the activity can be evaluated. Moreover peptides have got high antioxidant property. ISSN: 2637-4528

Isolation, purification and characterization of protein from Litchi chinensis honey and generation of peptides