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For the purpose of devising a colorimetric method for estimating the accurate amount of nitrite coexisting with various food additives, studies on a modified colorimetric method of Follett and Ratcliff have been made, and the following successful method has been developed in the present work.Since it has been found that reducing substances and hexametaphosphate interfere with the modified method of Follett and Ratcliff, potassium ferricyanide was added first to a model system containing nitrite and various food additives in order to oxidize the reducing substances.Potassium ferrocyanide formed by the reaction of potassium ferricyanide with reducing substances interfered with the modified method too, lead acetate, therefore, was added to precipitate it, whereupon hexametaphosphate was also precipitated by lead acetate simultaneously.By filtering off these precipitates, the filtrate came to be absolutely free from interfering substances, and accordingly the accurate amount of nitrite coexisting with various food additives could be estimated by determining the nitrite in the filtrate following the modified method of Follett and Ratcliff.

A Colorimetric Method for the Determination of Nitrite Coexisting with Various Food Additives