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In order to ensure compliance of raw bean paste products with the Japanese Food Sanitation Law, we examined the performance characteristics of an analytic procedure based on steam distillation followed by carbonate-pyrazolone assay to determine cyanogenic compounds in raw bean paste. The present method includes a procedure for decomposition cyanogenic glycosides using linamarase due to the possibility that cyanogenic glycoside-hydrolyzing enzymes might be deactivated in raw bean pastes. The performance of the method was evaluated using two types of bean paste spiked with a cyanogenic glycoside (linamarin) corresponding to cyanide ion concentrations of 5 and 10 mg/kg. The trueness of the method was 86-90%, and the repeatability (RSD) was 1.0-2.4%, while the intermediate precision (RSD) was 2.6% to 4.9%. A surveillance of cyanogenic compounds in 28 raw bean pastes manufactured in Japan was then carried out with the validated method. One sample contained 15 mg/kg (as hydrogen cyanide), while the other samples contained less than 5 mg/kg.

Evaluation of an Analytical Method for Cyanogenic Compounds in Bean Paste and Its Application for Surveillance of Real Samples