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A simple and rapid method using liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS) was developed for the determination of 25 kinds of sulfonamides in livestock products and seafoods. The sulfonamides were extracted with acetonitrile by glass bead homogenization and cleaned up with a tandem-connected ODS and basic alumina column. The quantification limits of 25 kinds of sulfonamides were 0.0025-0.005 microg/g. When two sulfonamides of specific samples were excluded, the recoveries and relative standard deviations were 70 to 120% and less than 15%. These results show that the developed method, which minimizes the matrix effect, offers high precision and should be useful for the determination of sulfonamides in livestock products and seafoods.

Determination of Sulfonamides in Livestock Products and Seafoods by Liquid Chromatography/Tandem Mass Spectrometry Using Glass Bead Homogenization