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A simple and rapid screening method using HPLC for the simultaneous determination of 13 veterinary drugs (oxytetracycline, quinoxaline-2-carboxylic acid, thiabendazole, 5-hydroxythiabendazole, albendazole-2-aminosulfone, sulfadimidine, flubendazole, zeranol, β-trenbolone, sulfadimethoxine, sulfamonomethoxine, oxolinic aid, and enrofloxacin) in meat and fish has been developed. The drugs were extracted with 0.25% metaphosphoric acidmethanol-acetonitrile (6:2:2, v/v), and the extracts were cleaned up on an OASIS HLB (60mg) cartridge. The HPLC separation was performed on a Wakosil-II-5C18 RS column (150×4.6mm i. d.) with a gradient system of 0.025mol/L phosphate buffer (pH 2.5)-acetonitrile as the mobile phase at a flow rate of 0.6mL/min. The drugs were detected with ultraviolet (UV) and fluorescence detectors with time programming. The calibration graphs were rectilinear from 1 to 20ng for each drug. The recoveries at levels of 0.02-0.1μg/g were 75.1-90.5%.

Rapid Screening Method for Residual Veterinary Drugs in Meat and Fish by HPLC