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Angiogenesis is a complex event mediated by several angiogenic factors released from cancer cells and immune cells. There is compelling evidence that mast cell accumulation among the peritumoral inflammatory infiltrates contributes to a favourable microenvironment for tumor development and metastasis. The present study aimed to evaluate the relationship between mast cells and microvessels by applying the double staining CD34/toluidine blue in cutaneous malignancies. Materials and Methods: The study consisted of 50 cases of cutaneous malignancies. Combined Immunohistochemical and Histochemical methods used to study the microvessels and mast cells. Results: A significant correlation between the mast cell count and microvessel density was observed in all 3 cutaneous malignancies. Statistical analysis of the linear correlation between MCD and MVD was also performed (P

international journal of clinical and diagnostic pathology

Significance of assessment of microvessel density (MVD) and mast cell density (MCD) by combined immunohistochemical and histochemical methods in cutaneous malignancies