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The ability to acoustically separate zooplankton species is an important requirement for ecological studies and to improve biomass estimates. In order to distinguish between Euphausia superba and other swarm-forming macroplankters we used a dual frequency echo-sounder (120 and 38 kHz) and echo-integrator during a series of Longhurst Hardy Plankton Recorder (LHPR) hauls near South Georgia. We compared the acoustic parameter Mean Volume Backscattering Strength (MVBS) according to the equation: DELTAMVBS (dB) = MVBS 120 kHz - MVBS 38 kHz. Mean values of DELTAMVBS for E. superba, Themisto gaudichaudii and E. frigida were 4.6, 9.7 and 15.6 dB, respectively, and were significantly different, allowing the 3 species to be separated acoustically

Differences in backscattering strength determined at 120 and 38 kHz for three species of Antarctic macroplankton