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Hardy Weinberg equilibrium (HWE) was assessed for each locus in each of the treatments using the ‘genhw’ package in Stata (StataCorp 2007). No treatments showed significant deviations from HWE at either locus. Values in Table S1 are p-values from Fisher exact tests of HWE. There was also no significant linkage disequilibrium (LD) observed between the Mpi and Gpi genotypes in any of the treatments. Pvalues below refer to Fisher exact tests of genotypic LD between Mpi and Gpi. mtDNA analysis

Physiological stress and the fitness effects of Mpi genotypes in the acorn barnacle Semibalanus balanoides