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The influence of cross-shelf gradients, within and among distance strata, on the otolith\udchemistry of the non-dispersing reef fish Acanthochromis polyacanthus was examined on the Great\udBarrier Reef. Most of the variation in elemental ratios (Mg/Ca, Cu/Ca, Sr/Ca, Ba/Ca) was at the level\udof sites within reefs; some broad patterns of otolith chemistry were found cross-shelf. Sr/Ca and\udBa/Ca ratios increased with distance from shore. Highest ratios of Mn/Ca and Cu/Ca were found at\udthe outermost shelf sites at Myrmidon Reef, but Mn levels were generally greatest within 45 km of\udshore. Elevated ratios of Ba/Ca at Myrmidon Reef were persistent over 3 yr and are likely indicative\udof upwelling, which is known to occur in the area. The Ba/Ca ratios were the highest ever reported\udin marine fish otoliths, and accuracy of our estimates was confirmed in a paired laboratory test. The\udrobustness of reef-wide chemical signatures in fish was weak, but unique signatures were found\udwithin reefs. High resolution elemental records could help to identify sites affected by upwelling and\udthe periodicity of upwelling events

Otolith chemistry of the non-dispersing reef fish Acanthochromis polyacanthus: cross-shelf patterns from the central Great Barrier Reef