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The ELISPOT immunoassay, where sdmples are spotted onto 0 45 pin pore-size nitro- cellulose and incubations are based on a BLOTTO/Tween system, proved satisfactory for detecting northern anchovy Engraulis mordax yolk in euphausiid Euphdusid pacificd guts. Polycloiid antiseruni to anchovy yolk was developed in male New Zealand rdbbits. The system detected 30 ndnnograms purified yolk protein with 15000 diluted antiserum and positively identified 75 % of cxperiinmtally- fed and frozen euphausiids

Use of an ELISPOT immunoassay to detect euphausiid predation on larval anchovy