English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Freeze-drying of bacteria requires the use of a cryoprotective agent. Skim milk, polyvinylpyrrolidone, dextran, sodiuln glutamate, sucrose, horse or calf serum are all routinely used. The effects of 7 suspending media, each containing 1 of these cryoprotective additives, were studied on 3 strains each of 3 fish pathogenic gliding bacteria (Flavobacterium columnare, Flavobacterium psychrophilum and Flexibacter marjtimus). The viability of preserved bacteria was evaluated by colony enumerat~on on Anacker and Ordal medium (Fa . columnare and Fa. psychrophilum) and on Difco marine medium 2216E (Fx. maritjmus), 48 and 7 2 h after inoculation. The data show the 3 bacterial species to be optimally preserved in suspending media containing D~fco Bacto Brucella Broth (2/3) supplemented with either horse or foetal calf serum (1/3). K E Y WORDS: Freeze-drying Cl-yoprotectron . Flavobacteri~~ni . Flexjbacter . Fish pathogen Three species of gliding bacteria are well recognized fish pathogens. Flavobacterium columnare (= Flexibacter columnaris = Cytophaga colun~nans; Bernardet et al. 1996) has been reported as a pathogen for many freshwater fish species throughout the world (Austin & Austin 1987). Flarrobacterium psychrophilum (= Flexibacter psychrophilus = Cytophaga psychrophila; Bernardet et al. 1996) is responsible for high mortality in salmonids, particularly in coho salmon Oncorhynchus kisutch in the USA and Canada (Holt et al. 1993) and in rainbow trout Oncorhynchus mykiss in Australia and several European countries (Schmidtke & Carson 1995). Flexibacter maritimus causes fish disease in sea farms in Japan (Wakabayashi et al. 1986). Scotland (Bernardet e t al. 1990), Spain (Alsina & Blanch 1993), and France (Bernardet et al. 1994). The

Freeze-drying of Flavobacterium columnare, Flavobacterium psychrophilum and Flexibacter maritimus