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An in vitro study of the attachment of Vibrio pathogens to tissues of the giant tiger prawn Penaeus monodon (Fabricius, 1798) was carried out using species-specific and genus-specific monoclonal antibodies with indirect FITCimmunofluorescence microscopy. The attachment of V. alginolyticus and V. parahaemolyticus to different tissues was always stronger than the attachment of V. anguillarum, and overall, these data are consistent with epizootiological studies. Strains of other Vibno species used in this study showed no evidence of the attachment.

Immunodetection of specific Vibrio bacteria attaching to tissues of the giant tiger prawn Penaeus monodon