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DNA inserts obtained from a cloning of the penae~d shrimp baculovirus, BP, were nonradioactively labelled with digoxigenin for use as gene probes to detect BP in paraffin-embedded, formalin/alcohol-fixed tissue sections using in sit11 hybridization. Results show that the probes detect BP in various species of shrimp from different geoglaphicdl dreas. The probes demonstrate no reaction to BP-negative tissue sections. The probes also demonstrate no reaction to tissue infected with other penaeid shrimp viruses.

Application of gene probes to detect a penaeid shrimp baculovirus in fixed tissue using in situ hybridization