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A study was conducted to evaluate the efficacy of 7 substances at potentiating a formalinlulled Aeromonas salmonicida bacterin in juvenile coho salmon Oncorhynchus kisutch. The substances were injected into the fish along with the bacterin. The fish were challenged 27 d later with viable A. salmonicida cells by 2 methods (cohabitation and immersion). The cumulative mortalities in each of the experimental groups was then determined. A significant and consistent increase in protection over the groups receiving only the A. sal~nonicida bacterin was observed with 3 of the substances tested. These were VitaStinl-Taito, lentinan and formalin-killed Renibacterium salmoninarum cells. One of these materials, VitaStim-Taito (a 8-1,3 glucan), showed particular promise for further studies.

Influence of seven immunostimulants on the immune response of coho salmon to Aeromonas salmonicida