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The humoral immune response to Egtved virus, the causative agent of viral haemorrhagic septicaemia (VHS) in rainbow trout Oncorhynchus mykiss, was investigated by means of the enzymelinked immunosorbent assay (ELISA), immunofluorescence (IF), and the 50 'X plaque neutralization test (50°/"PNT). Sera from fish immunized with virus under aquarium conditions as well a s sera collected from fish in farms with different VHS status were included In the experiments. ELISA proved to be more sensitive and less timeand material-consuming than either IF or 50°/~PNT Using Elisa, antibody to Egtved virus was found in 54 %I of fish from infected trout farms examined w h ~ l e only 16 % were positive by 50°/r,PNT The IF test had a sensitivity close to that of ELISA, but was less suitable for examination of large numbers of sera. Non-neutralizing antibodies tend to perslst longer after VHS infection than neutralizing antibodies. The kinetics of the antibody response was close to that prev~ously published. No anamnestic immune response was observed with any of the tests. We conclude that our ELISA is well suited for VHS surveillance.

diseases of aquatic organisms

Detection of rainbow trout antibody to Egtved virus by enzyme-linked immunosorbent assay (ELISA), immunofluorescence (IF), and plaque neutralization tests (50%PNT)