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NOTE A simple technique for accelerating the growth of the kidney disease bacterium Renibacterium salmoninarum on a commonly used culture medium (KDMZ) ABSTRACT: A simple technique for significantly accelerating the growth of Renibacteriurn salmoninarum (Rs), causa-tive agent of bacterial kidney disease in salmonids, is described and evaluated. The technique is based on the well-known phenomenon, referred to as 'satellitism' or 'cross-feeding', in which a fastidious organism is induced to grow by placing it on an agar culture medium next to a non-fastidious feeder or 'nurse' organism. In the present procedure , the nurse organism is actually a stock culture of Rs, a heavy suspension of which is drop-inoculated (25 p1 aliquot) onto the centre of a KDM2 culture plate. Samples suspected of containing Rs are peripherally drop-inoculated on the plate Growth of the nurse culture occurs rapidly and modifies conditions In the culture plate such that growth of Rs cells In the peripheral samples is greatly accelerated. The effect is most pronounced when the number of Rs cells in penpheral samples is low. The mechanism underlying growth acceleration was not investigated. Renibacterium salmoninarum (Rs), the agent responsible for bacterial kidney disease (BKD) in sal-monids, is a frustratingly, slow-growing organism. With samples containing low numbers of Rs cells, such as those derived from subclinical infections, incubation times of ca 6 wk are commonly required for visible growth of the pathogen on agar media. In fact, one group of researchers recently advised using incubation periods of 12 wk before considering samples negative for Rs (Gudmundsdottir et al. 1988). This slow-growing property of Rs has discouraged research on the pathogen and has led to the development of non-cultural techniques for its detection (Pascho et al. 1987). In this report, we describe a simple technique for significantly accelerating the growth of Rs. The technique is based on the well-known phenomenon, referred to as 'satellitism' (Salle 1954, p. 266-267) or 'cross-0 Inter-Research/Printed in F. R. Germany feeding' (Davis et al. 1973, p. 61), in which a fastidious organism is induced to grow by placing it on an agar medium next to an unrelated 'nurse' organism. In the present case, the nurse organism is actually a stock culture of Rs rather than another species of bacterium. The procedure suggested itself when it was observed that samples containing very low numbers of Rs cells always exhibited improved growth if they occurred on the agar medium adjacent to …

A simple technique for accelerating the growth of the kidney disease bacterium Renibacterium salmoninarum on a commonly used culture medium (KDM2)