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Amyloodinium ocellatum, a common dinoflagellate parasite of marine fishes, could be propagated in walking catfish gill (GIB) cell culture using a very simple salt solution consisting of a 1:l mixture of Hank's Balanced Salt Solution and artificial seawater. This culture system did not require the addition of serum or other additives, although significant amounts of trace metals were present as contaminants of the reagent-grade salts used to prepare the salt solution. The salt composition of the medium was important to parasite survival even while the parasites were feeding on the host. The best media for continuous propagation were proportionately higher in K, Mg, Ca, and SO, The relatively simple composition of this culture system may greatly facilitate the study of A, ocellatum because of its strong resemblance to natural environmental condihons.

Culture conditions affecting the in vitro propagation of Amyloodinium ocellatum