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A procedure using DEAE cellulose and hydroxylapetite was developed for the separation of Aeromonas salmonicida P1 protease, P2 protease, and trout cell-specific hemolysin (T-lysin) from supernatants of broth cultures. The different proteases were demonstrated using protease inhibitors, substrate specificities, and polyacrylamide gel electrophoresis with protease substrates in the gels. Isolated PA protease an T-lysin were shown to act independently in the complete lysis of rainbow trout erythrocytes in vitro

Separation and in vivo analysis of two extracellular proteases and the T-hemolysm from Aeromonas salmonicida