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Uptake of zinc by Hymenomonas elongata (Haptophyceae) was studied as a function of concentration in medium (from 25 to 600 yg I-') and of exposure time. Freundlich adsorption isotherms turned out to be a function of zinc concentration with saturation at values > 150 pg Zn 1 l . Zinc uptake at 150 pg 1-' as a function of incubation time also followed saturation curves. In the dark, cells contaminated for 24 h and 48 h with zinc took up significantly less zinc than those placed in the light. Darkness or treatment of algae with DCMU, a powerful inhibitor of photosynthesis, strongly reduced zinc uptake. Cells lulled by glutaraldehyde fixation took up significantly less zinc over 48 h than living cells. Zinc uptake by H. elongata is, thus, a light-dependent process, which seems to be linked to cell metabolism.

Uptake of zinc by cultured phytoplankters Hymenomonas elongata