English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

We studied the influence of methodology on the variability of bacterial direct counts and biomass estimates. Two frequently used fluorochromes, 4,6-diamidino-2-phenylindole (DAPI) and 3, 6-bis(dimethylamino)acridine (acridine orange (AO)), were applied to determine bacterial abun- dances and sizes along a vertical profile in a oligo-mesotrophic freshwater lake (Piburger See, Aus- tria). Only 90 ± 11% of AO stained bacteria were detected with the fluorochrome DAPI. On average, DAPI stained cells were half as large (48 ± 11% of mean cell volume) as cells stained with AO. The observed discrepancies in abundance and cell volumes were significantly related to community DNA synthesis rates, as suggested by lower differences at higher uptake rates of ( 3 H)thymidine. In addi- tion, a decrease in the relative DNA content cell -1 with increasing cell size was found in the bacteri- oplankton assemblage. Considering the staining properties of the 2 dyes, this may partially account for the observed differences in mean cell sizes. We summarized and evaluated most linear and allo- metric volume-to-carbon conversion factors published during the past 2 decades. Total bacterial bio- mass was estimated by applying several of these conversion factors to data sets determined from DAPI and AO stained preparations. Depending on the dye and conversion factor, bacterial biomass, averaged over the total water column, ranged between 5 µg C l -1 and 165 µg C l -1 . As a result of this comparison we recommend the use of allometric conversion formulae specifically elaborated for a

aquatic microbial ecology

Precision of bacterioplankton biomass determination: a comparison of two fluorescent dyes, and of allometric and linear volume-to-carbon conversion factors