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Intracellular of carbon, nitrogen and phosphorus content in phytoplankton cells is traditionally measured using concentrates containing thousands to millions of cells. In the present study, we used a nuclear microprobe (NMP) to determine C, N and P concentrations in single filaments of 3 cyanobacteria species: Anabaena sp., Nodularia spumigena and Aphanizomenon flosaquae var. klebahnii isolated from Baltic Sea water. Estimations of C, N and P content per cell were calculated and compared to the concentrations found using traditional bulk methods. No significant differences regarding the C, N and P cellular content were found between the 2 methods for each of the species tested (p < 0.05). From our results, we conclude that the NMP can be a useful tool for studying the elemental content of single phytoplankton cells

Composition of carbon, nitrogen and phosphorus in single cells of three filamentous cyanobacteria using nuclear microprobe and traditional techniques