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The abundance and nitrogen fixation rates of Trichodesmium sp. integrated down to the deep chlorophyll maximum as well as nitrogen fixation rates in size-fractionated discrete surface water samples were measured across the Mediterranean Sea. The abundance of Trichodesmium sp. was generally low ( 20°C and Trichodesmium sp. was present at a higher density (ca. 700 trichomes m -3 ). Across the basin, size-fractionated nitrogen fixation rates were low (<250 nmol N2 m -3 h -1 ). The highest rate was measured at a station characterized by relatively high concentrations of total dissolved P and dissolved Fe. High δ 15 N values (8.04 ± 2.03‰) of the particulate organic nitrogen were in accordance with the observed low water column nitrogen fixation rates across the Mediter- ranean Sea.

Low water column nitrogen fixation in the Mediterranean Sea: basin-wide experimental evidence