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Many stable isotope analysis (SIA) stud- ies aim to track protein, which is assimilated in animal tissues from their food sources, to assess feeding ecol- ogy, movements, and ontogenetic shifts of marine ani- mals. Lipids are known to be a potential source of bias because they are depleted in 13 C compared to 12 C. Al- though lipids are usually removed before SIA, there is a lack of standardized analytical protocols for this pro- cedure. We tested the effects of lipid extraction with 2 chemical solvents (chloroform-methanol and petro- leum ether) in the humeri of the loggerhead sea turtle Caretta caretta to establish a standard protocol for processing the bone collagen of marine animals and to develop a mathematical cor rection. In both lipid ex- traction treatments, δ 13 C values were higher than the control, but only lipid extraction with chloroform- methanol showed significant differences. By contrast, the δ 15 N values were not affected by lipid extraction treatments with either solvent. The linear regression between the C:Nbulk ratio and Δ 13 C was not significant, which does not support the assumption that there is a predictable relationship between the C:N ratio and lipid content. Nevertheless, a significant positive rela- tionship be tween Δ 13 C and δ 13 Cbulk was observed, but such a model is not recommended as a mathematical lipid correction method because the model efficiency had a negative value, which indicates that the mean value of δ 13 Cle is a better predictor than the model it- self. These results suggest that lipid extraction should be taken into account in SIA of bone collagen tissues for accurate δ 13 C determination.

Effects of lipid extraction on the isotopic values of sea turtle bone collagen