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Tissue from both solitary and aggregated serpulids Galeolaria hystrix and Spiro- branchus cariniferus from southern New Zealand was sequenced using 18S, histone H3 and cytochrome b in order to determine whether these differences in ecology and lifestyle reflect the existence of cryptic species. In both cases, all 3 phylogenetic trees unequivocally combined soli- tary and aggregated individuals into 2 monophyletic groups corresponding to the nominal spe- cies. Some combination of larval behaviour, adult attractants and biotic/physical environmental factors are likely to be the drivers of reef formation in these serpulid worms. A previously sequenced Australian specimen of G. hystrix is not in the same clade as the New Zealand samples and requires re-investigation.

Reef formation versus solitariness in two New Zealand serpulids does not involve cryptic species