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The principle of freezing semen is to maintain the survival of sperm in the long term (long term preservation). Thawing is the process of thawing frozen cattle semen before it is used in the artificial insemination process (IB). This thawing has a big influence on the state of sperm, especially on the integrity of sperm in semen, depending on the temperature and length of time. The choice of temperature and length of time thawing is good and right can prevent damage to spermatozoa. The purpose of this study was to analyze the effect of differences in temperature and the length of post thawing time on the viability of FH (Frisian Holstein) cow spermatozoa and to find the optimal temperature and length of thawing time in the IB process. The research method was experimental using RAK with 3 treatment groups and 2x replications. The research material used frozen semen of FH cattle from the Singosari Center for Artificial Insemination (BBIB). The observed variable was Viability with Eosin staining of 2%. Data analysis used the Factorial ANOVA test. The results of this study are the length of time thawing did not differ significantly namely P> 0.05, temperature 25 ° C 20 seconds: 66.30%, 25 ° C 30 seconds: 66.80%, 25 ° C 40 seconds: 63, 30%, 37 ° C 20 seconds: 65.00%, 37 ° C 30 seconds: 74.30%, 37 ° C 40 seconds: 69.00%, 40 ° C 20 seconds: 75.50%, 40 ° C 30 seconds: 65.50%, 40 ° C 40 seconds: 67.50%, the highest percentage of life (viability) of spermatozoa is at 40°C with a duration of 20 seconds with a value of 75.50%. Conclusions the higher temperature with the short thawing time can support the high viability.  Keywords: Bos taurus, Frozen semen, Thawing, Spermatozoa Viability ABSTRAK Prinsip pembekuan semen adalah untuk mempertahankan kelangsungan hidup sperma dalam jangka waktu yang lama (long term preservation). Thawing adalah proses pencairan kembali semen beku sapi sebelum digunakan dalam proses inseminasi buatan (IB). Pencairan ini memiliki pengaruh besar pada keadaan sperma, terutama pada integritas sperma dalam semen, tergantung pada suhu dan lamanya waktu. Pemilihan suhu dan lama waktu thawing yang baik dan tepat dapat mencegah kerusakan spermatozoa.   Tujuan penelitian untuk menganalisa pengaruh perbedaan suhu dan lama waktu post thawing terhadap viabilitas spermatozoa sapi FH (Frisian Holstein) dan mencari suhu dan lama waktu thawing yang optimal dalam proses IB. Metode penelitian dilakukan secara eksperimental menggunakan RAK dengan  3 perlakuan  kelompok dan 2x ulangan. Bahan penelitian menggunakan semen beku sapi FH dari Balai Besar Inseminasi Buatan (BBIB) Singosari. Variabel yang diamati adalah Viabilitas dengan pewarnaan Eosin 2%. Analisis data mengguakan uji ANOVA Faktorial. Hasil penelitian diperoleh lama waktu thawing tidak berbeda secara signifikan yaitu P>0,05, suhu 25°C 20 detik : 66,30%, 25°C 30 detik : 66,80%, 25°C 40 detik : 63,30%, 37°C 20 detik :65,00%, 37°C 30 detik :74,30%, 37°C 40 detik : 69,00%, 40°C 20 detik : 75,50%, 40°C 30 detik : 65,50%, 40°C 40 detik : 67,50%, presentase hidup (Viabilitas) spermatozoa tertinggi terdapat pada suhu 40°C dengan lama waktu 20 detik dengan nilai 75,50%. Semakin tinggi suhu dengan waktu thawing yang singkat dapat mendukung tingginya viabilitas. Kata Kunci: Bos taurus, Semen beku, Thawing, Viabilitas Spermatozoa

Analisis Viabilitas Spermatozoa Sapi Friesian Holstein (Bos taurus) Post Thawing Semen Beku Dengan Pengaruh Suhu dan Lama Waktu Thawing Berbeda