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Mutations in the quinolone resistance-determining regions (QRDRs) of Acinetobacter baumannii DNA gyrase ( gyrA ) and topoisomerase IV ( parC ) are linked to fluoroquinolone (FQ) resistance. We developed a mismatched PCR-restriction fragment length polymorphism (RFLP) assay to detect mutations in the gyrA and parC QRDRs associated with FQ resistance in A. baumannii .

annals of laboratory medicine

A Novel Mismatched PCR-Restriction Fragment Length Polymorphism Assay for Rapid Detection of gyrA and parC Mutations Associated With Fluoroquinolone Resistance in Acinetobacter baumannii