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Peptide nucleic acid (PNA) probes are artificial DNA analogues with a hydrophobic nature that can penetrate the mycobacterial cell wall. We evaluated a FISH method for simultaneous detection and identification of Mycobacterium tuberculosis (MTB) and non-tuberculous mycobacteria (NTM) in clinical respiratory specimens using differentially labeled PNA probes.

annals of laboratory medicine

Evaluation of Dual-Color Fluorescence <i>In Situ</i> Hybridization With Peptide Nucleic Acid Probes for the Detection of <i>Mycobacterium tuberculosis</i> and Non-Tuberculous Mycobacteria in Clinical Specimens

Evaluation of Dual-Color Fluorescence In Situ Hybridization With Peptide Nucleic Acid Probes for the Detection of Mycobacterium tuberculosis and Non-Tuberculous Mycobacteria in Clinical Specimens