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Elicitation strategies were studied for yield enhancement of colchicine, produced by root cultures of Gloriosa superba . Adventitious root cultures were established and grown in media containing 3 mg/l NAA and 1 mg/l BA. Root cultures showed variations in biomass as well as colchicine production in the presence of different exogenous carbohydrates and amino acid. Among the different sources of carbohydrates used - fructose, sucrose and dextrose gave a substantially higher biomass yield than the control. Maximum biomass was obtained in the presence of fructose. Root cultures growing in mannitol supplemented medium resulted in maximum accumulation of colchicine (0.32%). Among the amino acids, serine and phenylalanine significantly enhanced colchicine accumulation in root cultures. 0.02 mM glutamine supplemented media showed maximum (ten-fold) increase of root biomass. Plant Tissue Cult. & Biotech. 25(2): 247-256, 2015 (December)

Role of Exogenous Carbohydrate and Amino Acid Sources on Biomass and Colchicine Production in Nontransformed Root Cultures of Gloriosa superba