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The microbial activity of ten selected Azospirillum isolates was measured in terms of the amount of CO 2 evolved by the isolates after incubation for 5 days which ranged from 6.88 to19.25 mg. Nitrogen fixing efficiency all of the isolates was determined by microkjeldhal method and the nitrogen fixing efficiency ranged from 10.03 to 13.11 mg N/g substrate. The growth of Azospirillum isolates was significant affected by different physiological factors such as pH, temperature and salinity. Most of the isolates showed optimum growth at pH 6.8, temperature 37°C and in the absence of NaCl. Azospirillum has the potential to be used as a substitute and or supplement of N-fertilizers. Further research is needed to estimate N-supplement potentials of biological nitrogen fixation (BNF) systems at the farm level. Jahangirnagar University J. Biol. Sci. 3 (1): 47-53, 2014 (June)

Nitrogen fixing efficiency and physiological characteristics of Azospirillum isolates from the paddy fields of North Bengal