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In an investigation of Newcastle disease (ND) vaccination programme, the performance of ND killed vaccine as a secondary one was performed to examine duration and level of antibody in both broiler and layer breeder parent stock in the Department of Microbiology and Hygiene, BAU, Mymensingh during the period from January to April 2004. For this, a total of 210 broiler and layer breeder birds of seven different breeds and ages flocks with history of regular vaccination were selected. A total of 30 breeder birds were selected for each of the experimental group. Birds, each of seven flocks were vaccinated with commercially availably two ND killed vaccine named as "Newcevac Nobilis®" and "Nobivac ND broiler®", Intervet. The Lohman Brown (LB-31), BV-300 (B-36), BV-300 (B-22) and Lohman Brown (LB-24) layer breeder birds were vaccinated with Newcevac Nobilis® killed vaccine @ 0.5ml/bird by intra-muscular route at 28, 25, 16 and 19 weeks of age and blood samples were collected at the age of 31, 36, 22 and 24 weeks and showed mean HI antibody titer ± SD 1518.93 ± 593.50, 563.20 ± 303.65, 1177.60 ± 618.36 and 1604.26 ± 655.04 respectively. In case of broiler breeder parent stock, Kasila (K-16), Kasila (K-34) and CoBB (Co-17) which were vaccinated with Nobivac ND broiler® killed vaccine @ 0.2ml/bird by intra-muscular route at 13, 24 and 13 weeks of age and sera samples were collected at 16, 34 and 17 weeks of age respectively which showed mean HI antibody titre ± SD 1117.87 ± 670.55, 725.33 ± 315.93 and 1109.33 ± 670.04 respectively. Considering the result of the study, vaccination with ND killed vaccine provoked a high level of humoral immunity and such a high level of antibody, as were observed, could be useful for breeder farms where chicks are required to have higher maternal antibody during their chick hood.

Investigation on the immunity level of breeder flocks following vaccination with Newcastle disease virus vaccine