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This study was carried out to investigate the potential of Corn (Zea mays) for phytoremediation of soil contaminated with lead (Pb) and zinc (Zn). The Zea mays L. cv. Giza 2, Hordeum vulgare cv. Giza 123 and Lupinus termis cv. Giza 1 species were planted in poted soil contaminated with lead and zinc. Zea mays was also cultivated with Hordeum and Lupinus treated with lead and zinc. The results showed that growth decreased by (16.7, 48.63 and 23.56%), photosynthetic pigments decreased by (10.18, 22.38 and 10.9%) and total-N decreased by (30.0, 27.27 and 13.64%) in Zea, Hordeum and Lupinus, respectively as compared to control. Proteins profile in shoots of tested plants revealed qualitative and quantitative changes. Co-cultivated Zea with Hordeum and Lupinus treated with lead and zinc improvement all parameters. The evidences provided by this experiment indicated that Corn acts as an effective accumulator to zinc and lead . Introduction High concentrations of heavy metals in soil may affect crop growth, as these metals interfere with metabolic functions in plants, including physiological and biochemical processes. The most dangerous heavy metals are Pb, Hg, As, Cd, Sn, Cr, Zn and Cu (Wright 2007 and Gosh 2010). Zn2+ at high levels leads to chlorosis in young leaves, inhibits photosynthesis at various steps and decreases the growth and metabolic activity in various plant species (Vaillant et al., 2005). Moreover, the reduction in nitrogen content under high zinc treatment had been shown by Sivasankar et al. (2012). Pb2+ inhibits some of metabolic activities in the plant (Hamid et al., 2010). Eun et al. (2000) showed that a reduction in growth when exposed to increased levels of Pb resulted in the inhibition of cellular processes within root tips. In addition, responses of plants to Pb exposure include a decrease in root elongation and biomass (Fargasova 2001). Also photosynthesis is adversely affected by increasing levels of Pb through the distortion of chloroplasts, inhibition of electron transport, reduction of chlorophyll synthesis, disruption of Calvin’s cycle enzymes and a reduction in carbon dioxide intake (Sharma and Dubey 2005). Saygideger et al. 2004, showed that there was a decrease in total-N content of Typha latifolia in parallel to increased Pb concentration. Plants that accumulate metals to high concentrations are sometimes referred to as ‘‘hyperaccumulators’’ (Visoottiviseth et al. 2002). Corn (Zea mays) is known to be a good accumulator of contaminants (Molgarzata and Andzej 2005). The maize plant has been even shown to accumulate certain heavy metals such as Pb (Pereira et al. 2007). The aim of this study is to assess the effect of lead and zinc on physiological and biochemical attributes of Zea mays L. cv. Giza 2, Hordeum vulgaris cv. Giza 123 and Lupinus termis cv. Giza 1, and the potential of Zea mays for phytoremediation of lead and zinc. Material and Methods Pure strains of Zea mays L. cv. Giza 2, Lupinus termis cv. Giza 1 and Hordeum vulgare cv. Giza 123 were obtained from Egyptian Agricultural Organization, Ministry of Agriculture, Egypt. *Author for correspondence: <hananhelmy70@yahoo.com>)

Phytoremediation of soil contaminated with zinc and lead by using Zea mays L.