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This study aimed to evaluate the effect of the presence and absence of light provided to the mother plant and zeatin concentrations in vitro establishment, to reduce oxidation and promote the establishment of olive explants. The trials were carried out at the Fruit Tree Propagation Lab, Federal University of Pelotas, Brazil. In the first experiment, plants of olive 'Arbequina' were subjected to the absence and presence of light and established on WPM with 0 and 2mg.L-1 zeatin. In the second experiment, plants of olive 'Leccino', 'Arbequina' and 'Frantoio' 'were submitted to the absence of light, and established in vitro on WPM with 0,4 and 8 mg.L-1 Zeatin. Mother plants of olive tree 'Arbequina' in conditions of absence of light are the most suitable in vitro establishment. The concentration of 2 mg.L-1 of zeatin promotes survival of explants of olive 'Arbequina'. The concentrations 0,4 and 8 mg.L-1 of zeatin) results no difference between Leccino and Arbequina cultivars for the variable fungal contamination.

Mother plant luminescence and zeatin concentration in the in vitro establishment of an olive plant