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Minnie root (Ruellia tuberosa L .) belongs to Acanthaceae family. In traditional medicine has been used as diuretic, antidiabetic, antipyretic and antihypertensive and it also recently been incorporated as a component in a herbal tea in Taiwan. This research is aim to determine LC 5 0 value of Minnie root leaves extract (Ruellia tuberosa L .) to Artemia salina Leach shrimp larvae. Dryed samples extracted by graduate maceration with n-hexan, ethyl acetate and ethanol respectively. Rendamen n-hexane extract obtained as much as 1.158%, ethyl acetate extract as much as 2.017% and ethanol extract as much as 3.669 %. Based on qualitative screening used TLC method, ethyl acetate extracts and ethanol extracts of Minnie root (Ruellia tuberosa L .) leaves contain alkaloid, saponin, flavonoid and phenolic compounds. Toxicity assay Artemia salina Leach shrimp larvae have been conducted on leaves extracts of Minnie root (Ruellia tuberosa L .). Toxicity assay on sample extract of Minnie root (Ruellia tuberosa L. ) leaves determine by counting the number died of larvae after 24 hours treatment. LC 5 0 on sample calculated by probit analysis method. The toxicity assay showed LC 5 0 of ethyl acetate extracts and ethanol extracts are toxic with LC 5 0 266.07 ± 0.93 µg/mL and 142.160 ± 1.30 µg/mL.

UJI TOKSISITAS EKSTRAK DAUN PLETEKAN (Ruellia tuberosa L.) DENGAN MENGGUNAKAN METODE BRINE SHRIMP LETHALITY TEST (BSLT)